During compared with the reaction luminol experiences with Fe+3

During an interconversion
between the keto and enolic forms occurs whilst luminol is dissolved in a
strongly basic solution, it was discovered that the keto form experiences both
chemiluminescence, when in the presence of oxidising agents, and fluorescence
while the enol form does not emit light as a result of a chemiluminescence or
fluorescence. The behaviour of luminol is entirely dependent on the environment
the molecule is in as a hypochlorite constituted bleach acts as an interfering
agent (9) and when
used in a crime scene scenario will emit blue light for a period of 30 seconds
comparable to the way it reacts when luminol comes into contact with Fe+3 and
catalytic hydrogen peroxide. At first a considerable reduction in the intensity
of blue light will be observed, when compared with the reaction luminol
experiences with Fe+3  and
catalytic hydrogen peroxide, but after a short period it will be decidedly
difficult to differentiate between their respective chemiluminescent intensity (10). This is one example of a false positive due to the possible presence
of metal ions, peroxidases, urine and cleaning agents (9). Luminol
is a common tool employed in a crime scene to detect blood stains. Several proposals
have been made to reduce the luminol photon emission with interfering
substances in order to ensure the crime scene isn’t tampered with by employing
the use of  primary and secondary amines (11). Primary and secondary amines have been associated with acting as a
hindrance to chemiluminescent when luminol is interacting with anything other
than iron in blood. The purpose of this is to quench the reaction so a
determination may be made if blood is present.

RR’NH + HOCl => RR’NCl + H2O

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Reaction 1: Primary
or secondary amines interacting with hypochlorous acid where (R,R’=alkyl or H)

R3N + HOCl => R3NCl + H2O

Reaction 2:
Tertiary amines interacting with hypochlorous acid to yield chlorotrialkylammonium 

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